Coalition’s letter to Union Agriculture Minister on two untested and unsafe gene edited rice varieties

From: GM Free India <indiagmfree@gmail.com>
Date: Thu, 26 Jun 2025 at 12:00
Subject: Sub: Urging you to not release untested and unsafe gene edited rice varieties, Kamala and Pusa DST Rice 1, gene-edited sheep and to urgently bring all kinds of gene editing under the purview of comprehensive and rigorous regulation
To: <agrimin.india@gmail.com>, <chauhansr@mpls.sansad.in>
Cc: <bhupenderyadav69@gmail.com>, <bhupender.yadav@sansad.nic.in>, <mefcc@gov.in>, <mos-stes@gov.in>, <minister-fahdpr@nic.in>, <lalanrrsingh@yahoo.co.in>, <lalanrajiv1@gmail.com>, <rrsingh@mpls.sansad.in>

To:

Shri Shivraj Singh Chauhan

Hon’ble Union Minister for Agriculture & Farmers’ Welfare

Government of India.

Email: agrimin.india@gmail.com, chauhansr@mpls.sansad.in

Dear Shri Shivraj Singh Chouhan ji,

Sub: Urging you to not release untested and unsafe gene edited rice varieties, Kamala and Pusa DST Rice 1, gene-edited sheep and to urgently bring all kinds of gene editing under the purview of comprehensive and rigorous regulation

Namaste. At the outset, we would like to introduce ourselves. “Coalition for a GM-Free India” is a national volunteer-driven platform of hundreds of public-spirited organisations and individuals working on issues related to agriculture, health, conservation of biodiversity, appropriate technology, food safety and empowerment of farmers. We are deeply concerned about the regulatory deficiencies in India when it comes to gene technologies and their deployment in our farming systems/environment and realise that these affect citizens, their health, livelihoods and environment. We are grateful to you for expressing your concern with regard to multiple adverse impacts of transgenic GM crops and thank you for your wisdom and long-term vision on this matter. 

We write this letter to you to express our deep concerns with regard to gene edited crops, and share the scientific information that we have in this regard. We believe that you have been misled by a particular “scientific community” (that for decades has sought to promote transgenic crops) ignoring scientific literature on the imprecision and unpredictability of gene editing, in addition to other related issues. In particular, we would like to draw your attention to unanswered questions with regard to the two announced gene edited rice varieties Kamala and Pusa DST Rice 1. Even though this letter is long, we request your patience in going through all the materials we are sharing here.

Gene Editing is imprecise:

Although the agricultural biotech industry and its allies claim that gene-editing tools such as CRISPR-Cas bring changes in the genome in a precise and controlled way leading to specific intended changes and nothing else, there is a large and ever-growing number of scientific evidence showing that gene editing is not precise but gives rise to numerous genetic changes, also known as unintended mutations both at the targeted site and off-site. These unintended mutations in SDN-1 technology used in the two gene edited rice varieties that were announced in May 2025, can lead to the creation of new gene sequences producing new mutant proteins, with unknown consequences to the health of consumers, and to the environment. These alterations can lead to compositional changes, which, scientists warn, could prove to be toxic and/or allergenic to human or animal consumers. We attach here a list of illustrative studies that capture numerous unintended changes from this so-called precise breeding technique. 

Unreliability of CRISPR-Cas application:

There are several scientific papers cautioning us about the irreversible risks associated with CRISPR technology, the same technology used in the two recently announced gene-edited rice varieties. We attach some illustrations of scientific evidence on the same. 

Gene Editing technique uses foreign genetic material, and such alien materials get unintentionally inserted into the new variety:

The process of creating genome edited organisms involves the use of foreign genetic material, in the form of plasmids/other vectors, antibiotic resistant marker genes, promoter genes etc, even in cases where the intention is not to insert alien or exogenous genetic material. It is important to note that de-regulated techniques of SDN-1 and SDN-2 gene editing also involve foreign genetic material being used and unintentionally implanted in the host organism. Since SDN-1 and SDN-2 gene editing tools have been de-regulated, there is no biosafety testing mandated at the moment in India to check for the inclusion of such foreign genetic material used in the process. We attach illustrations of scientific evidence on the unintentional inclusion of foreign genetic material in gene editing, even where the intention is not to introduce alien genes (SDN-3). 

IPR issues:

Gene editing tools are proprietary technologies under IPR ownership and have a direct bearing on seed sovereignty of India’s farming community, in addition to potentially being a wastage of public resources (like we witnessed in the infamous case of Bikaneri Bt cotton, for example). The Government of India has not revealed the situation with regard to IPRs on the announced varieties. There is lack of clarity on where the Indian Government’s NRAS picked up the technological tools from, for developing these two rice varieties, on what terms and conditions. While Broad Institute has been mentioned in news articles, this Institute has patents on the technology in the USA which are under litigation. In India, University of California and University of Vienna are patent-holders, which has met with post-Grant Opposition in India recently. 

Threat to India’s export markets for rice:

India is the world’s largest producer of rice at present. It is obvious that the surplus rice needs global markets. Meanwhile, important global markets have legitimate reservations about gene edited rice (these varieties would be GM with the foreign genetic material being in them too) and these markets reject such rice. We are jeopardising our trade security from these two genome edited rice varieties. More importantly, countries in the Middle East/Gulf, which are major destinations for our rice exports, prefer organic and public sentiment is against GM.

Other concerns around India’s traditional rice diversity, natural farming and other matters: 

These two gene-edited rice varieties put India at risk of losing its diverse gene pool with respect to rice. You might recall the permanent and irreversible damage to traditional cotton varieties and their germplasm with GM cotton’s arrival on our farms even as research from leading cotton scientist, ex-CICR director Dr Keshav Kranthi has shown that GM cotton did not lead to promised yield increase. India is a Centre of Origin and Diversity for rice, and it becomes imperative that India protects our rice diversity pool especially in the age of climate change. Risk to these traditional varieties threatens natural farming too – for which traditional varieties are key. Within these traditional varieties which will be at risk also lie a number of traditional drought and salinity tolerant varieties as well as high yielders, with good nutritional characteristics.

Rice is used as akshat by many communities in India. “Akshat” means unbroken and whole. Here however, 366 base pairs were deleted through gene editing to develop Pusa DST Rice 1 whereas 33 base pairs were deleted for DRR Dhan-100 “Kamala”. This destroys the sanctity of rice to be used in various rituals and ceremonies.

Claims that rice cultivation area can be reduced through these two varieties unfounded and baseless:

To give a cover of acceptability to these two gene edited rice varieties, the Government of India is presenting a narrative that the increased productivity of these rice varieties will lead to reduction in rice cultivation area, freeing up land for cultivation of pulses and oilseeds. The implausibility of this claim is apparent to the discerning person; clearly, it is not lack of productivity that has increased the area under paddy cultivation in India but perverse/locked-in incentives tied to paddy production. 

Meanwhile, we also have higher productivity in both traditional and conventionally-bred rice varieties than the gene edited rice varieties. Rather than these gene-edited rice varieties, the Government of India should be promoting such safer rice varieties.

Sir, it is important to recall that a deliberate ploy has been deployed with a plan to release these varieties without due testing and regulatory oversight, by de-regulating SDN-1 and SDN-2 techniques. We emphasise that IBSC oversight is equivalent to de-regulation, given the obvious conflict of interest involved. In other words, as per changes made in the statutory regulations governing gene editing in India in 2022, Institutional Bio-Safety Committees (IBSC) are supposed to self-regulate SDN-1 and SDN-2 R&D, wherein IBSC only informs the Review Committee on Genetic Manipulation (RCGM) in the Department of Biotechnology while the IBSC is the one which reviews documents submitted and even issues a certificate of exemption to the applicant within the institute. This is a clear conflict of interest. Why will any institute (and its IBSC) stop its own employee from proceeding forward in the R&D? This is equivalent to de-regulation.

Meanwhile, countries like Mexico, Malaysia, South Korea and Indonesia clearly cover all genome edited products under statutory regulatory regimes. Even in the USA, towards the end of 2024, a Federal Court has ruled that regulatory frameworks are applicable to gene edited plants. Further, countries in the Middle East, which are major export destinations for India’s rice, continue to reject GM produce, including genome edited products as some consumer surveys are revealing. 

Given the many concerns we have mentioned in this letter, and the compelling scientific evidence presented herein, there is an immediate need to ensure the 2 gene edited rice varieties – Kamala (DRR Dhan 100), Pusa DST Rice 1 and gene-edited sheep are not released. 

If gene-edited rice varieties and sheep are indeed as safe and beneficial as they are claimed to be, let them be proven so after undergoing thorough testing with data that is publicly shared for wider scientific scrutiny. 

We urge you to – 

1)  Ensure that the planned release of Kamala (DRR Dhan 100), Pusa DST Rice 1 and gene-edited sheep is withdrawn immediately, and share all data related to Kamala (DRR Dhan 100), Pusa DST Rice 1, gene-edited sheep (and subsequently for all gene edited crops and animals) in the public domain.

2) Ensure that all gene editing in agriculture and animals remain fully regulated under GMO regulations given the inherent imprecision of gene editing and detailed reasons shared above. Exemption of SDN-1 and SDN-2 category gene-edited plants under Rule 20 of the “Rules for the manufacture, use, import, export & storage of hazardous microorganisms/genetically engineered organisms or cells, 1989” must be withdrawn. 

Sincerely, 

Kavitha Kuruganti                                      Rajesh Krishnan

Co-Convenor                                             Co-Convenor

Copies to – 

1) Shri Bhupender Yadav, Union Govt Minister for MoEFCC

2) Dr Jitendra Singh, Minister of State (Independent Charge) for Science and Technology

3) Shri Rajiv Ranjan Singh, Union Govt Minister for Animal Husbandry and Dairying

Letter Endorsed by:

  1. Dr. A. R. Vasavi, Independent Researcher, Bengaluru

  2. Dr. Adv Lubna Sarwath, Center for Environmental & Wellbeing Economics, Hyderabad

  3. Dr. Agastin B, Retd. Professor, Tirunelveli, Tamil Nadu

  4. Dr. Aishwarya J, Independent Researcher, Chennai, Tamil Nadu

  5. Dr. Aishwarya P, BNYS Physician, Bangalore

  6. Dr. Anand Dhananjay Padhye, Professor and Head (Retired), Pune

  7. Dr. Anupam Paul, Kolkata, West Bengal

  8. Dr. Arun Gupta, Pediatrician and Public Health Expert, Delhi, Delhi NCR

  9. Dr. Avik Ray, Researcher and Analyst, Kolkata

  10. Dr. Balaji Krishnasamy, Doctor and Farmer, Puducherry

  11. Dr. Bharat Shah, Doctor, Vadodara

  12. Dr. Burnad Fathima Natesan, Tamil Nadu Dalit Women’s Movement

  13. Dr. D. K. Sadana, ILSI, Karnal, Haryana

  14. Dr. Debal Deb, Conservative biologist, Centre for Interdisciplinary Studies, Kolkata

  15. Dr. Debarati Chakraborty, Agri-research, Hyderabad

  16. Dr. Debasis Laha, Writer, researcher, Belgharia, Kolkata

  17. Dr. Deepa Ramesh, Velan Hospital, Tirupur

  18. Dr. Devendra Jani, Pune, Maharashtra

  19. Dr. Dhanya K, Yoga Instructor (Contract NAM), Kerala

  20. Dr. Dheeraj D S, Ayurvedic doctor, Kottayam

  21. Dr. Dhiraj Singh, Retired Agriculture Scientist, Ganganagar and Meerut

  22. Dr. Dipankar Dey, Teacher, Kolkata

  23. Dr. Donthi Narasimha Reddy, Seed Campaign, Hyderabad

  24. Dr. Dwiji Guru, The Millet Foundation, Sitapur (UP) / Bengaluru (Kar)

  25. Dr. Felix Joseph X, Professor, Kanyakumari ,Tamilnadu

  26. Dr. G.Sither, Naturopathist, Thanjavur

  27. Dr. Gautam Das, Individual, West Bengal

  28. Dr. Gopal Krishna, ToxicsWatch Alliance (TWA), New Delhi

  29. Dr. Gunathilagaraj Kandasamy, Retired Professor of Agricultural Entomology, Coimbatore

  30. Dr. Gunjan Sharma, Yoga Vahini, Gurgaon

  31. Dr. H Sumanth Hegde, Doctor, Bangalore Karnataka

  32. Dr. Harish Gopal Shetty, Nature Cure, Andheri, Munbai

  33. Dr. Hemang C Shah, Doctor at Ayush Vision Care Eye Clinic, Kutch, Gujarat

  34. Dr. Jegath Krishnan G, Doctor, Kollam

  35. Dr. Kanchan Mathur, Gender Specialist, Jaipur

  36. Dr. Kannadasan, Farmer, Tamilnadu

  37. Dr. Karthik Subramanian Chandrasekaran, Senior Molecular Cell Biologist, Chennai

  38. Dr. Kavita Kulkarni, Professor, Pune

  39. Dr. Kranthi Kumar Reddy, National Oilpalm Farmers Association, Pragadavaram, Andhra Pradesh

  40. Dr. Krithika, Former DST Woman scientist (Wos-A), Chennai, Tamil Nadu

  41. Dr. Kumar Gupta, Physician, Mumbai, MA

  42. Dr. Lalitha Vijayan, Honorary Director, Salim Ali Foundation, Kanimangalam, Thrissur

  43. Dr. M.Sivapragash, Professor, Nagercoil

  44. Dr. Madhulika Banerjee, University of Delhi, Delhi

  45. Dr. Mita Dutta, Centre for Interdisciplinary Studies, Kolkata

  46. Dr. Mohammad Ismail Gulshath, AINDIAA, Kovilpatti, Tamil Nadu

  47. Dr. Mohan Rao, Former Professor of Public Health, Bangalore

  48. Dr. Murali KV, Medical Doctor cum Doctorate in Medicine, Bengaluru

  49. Dr. Nandita Shah, Doctor, Mumbai

  50. Dr. Nanthini R O, Chennai

  51. Dr. Nilanjan Ghosh, Musician, Kolkata

  52. Dr. Padmini Raghavan, Member of Obstetric and Gynaecology Society And of Nature Lovers of Hyderabad, Secunderabad, Telangana

  53. Dr. Padmini Ranganathan, Ayurveda doctor, Mysore

  54. Dr. Partha Bhore, Assistant Professor, Howrah

  55. Dr. Parthiban Shanmugam, Public Health Consultant, Chennai

  56. Dr. Pooja Mukul, Enable Foundation for Persons with Special Needs, Jaipur

  57. Dr. Prabir Chatterjee, Doctor, Phulberia, Bankura, W Bengal

  58. Dr. Prableen Kaur Chatha, Doctor, Jaipur

  59. Dr. Pradeep Kumar, Medicine, Chennai

  60. Dr. Prasanta Chattopadhyay, Kalodhvani, Belgharia, Kolkata

  61. Dr. Prof. B.Narsimha Reddy, Retd. Professor of Botany, Hyderabad,Telangana

  62. Dr. Pushya A Gautama, Doctoral Scholar, Bangalore

  63. Dr. Raamnath ARR, Dot School of Design, Chennai

  64. Dr. Radhika, Teacher, Dharapuram

  65. Dr. Rakesh Agrawal, Independent Researcher & writer, Dehradun, Uttarakhand

  66. Dr. Ramakrishnappa, Belavala Foundation, Srirangapatna

  67. Dr. Raman, Professor, Madurai

  68. Dr. Regi M George, Doctor, Sittilingi, Tamil Nadu

  69. Dr. Rekha J Gowda, Ayurvedic doctor, Mysore

  70. Dr. Rupal Shah, Green Leaf Ayurved Clinic, Pune

  71. Dr. S Rajeshwaran, Farmer, Virudhunagar

  72. Dr. S. Murugesan, Indian Organic Farms Association ( IOFA), Chennai

  73. Dr. S.Santhi, Freelance Ecolgical Educator, Trivandrum, Kerala, India

  74. Dr. Safia Aggarwal, Land and forest governance, Whitefield, Bangalore

  75. Dr. Salauddin Baig, Service, Jamshedpur

  76. Dr. Sanjay Agrawal, Doctor, Jaipur, Rajasthan

  77. Dr. Sanjit Chatterjee, Research and teaching, Bangalore

  78. Dr. Sarath Babu Balijepalli, President, Hyderabad

  79. Dr. Sarita Kapgate, Ayurveda, North Goa

  80. Dr. Selvan Rathinasamy, Child doctor, Erode

  81. Dr. Shajehan SIvasankara Pillai, Retired Neurosurgeon, Trivandrum

  82. Dr. Shakeelur Rahman, Prakriti Bachao Foundation, Ranchi

  83. Dr. Shukla, Maharashtra

  84. Dr. Shyamal Samanta, Agriculture, Genetics and Plant Breeding, Howrah

  85. Dr. Siddhartha Gupta, Asst professor, Kolkata

  86. Dr. Sita Venkateswar, Massey University, New Zealand/Kolkata

  87. Dr. Smily, MAA Ayodhya Seva Sansthan, Uttar Pradesh

  88. Dr. Smita Mandlecha, Professional, Pune

  89. Dr. Soma Marla, Retired Principal Scientist, Hyderabad

  90. Dr. Sreeraj K Damodar, AMAI, Varappetty

  91. Dr. Sudeshna Sengupta, Independent Researcher and Consultant, Gurugram, Haryana

  92. Dr. Suhas Kolhekar, Molecular Biologist & Health Rights Activist, Pune, Maharashtra

  93. Dr. Sujatha Byravan, Biologist, Chennai

  94. Dr. Sunilam, Kisan Sangharsh Samiti, Parmandal

  95. Dr. Sushant Satpathy, Consultant Retired, Bhubaneswar

  96. Dr. Tanmoy K Pal, Academic Administration, Kolkata

  97. Dr. Tarak Kate, Dharamitra, Wardha

  98. Dr. Taru Veera Venkata MaruthiSuman, Freelancer, Dharwad, Karnataka

  99. Dr. Uma Shankari, Farmer, Hyderabad, Telangana

  100. Dr. Umesh Chandrasekhar, Professor of Management, Coimbatore

  101. Dr. US Natarajan, Srivilliputtur

  102. Dr. Usha R, Doctor, Thiruvananthapuram

  103. Dr. V. S. Vijayan, Chairman, Salim Ali Foundation, Kanimangalam, Thrissur, Kerala

  104. Dr. V.G. Udayakumar, Ayurveda Medical Association of India, Malappuram, Kerala

  105. Dr. Veena Shatrugna, Scientist Clinical Nutrition, NIN, (Rtd), Bengaluru, Karnataka

  106. Dr. Vijay Rukmini Rao, Development,  Hyderabad, Telangana

  107. Dr. Vijay Sandeep Jakkula, RySS, Thematic Lead, Science & Research, Hyderabad

  108. Dr. Xaviar Rajappa, Retired Head Master, Sivagangai

  109. Dr. Yatin Diwakar, Development professional and evaluator, Navi Mumbai

  110. Mr. A Basu, Kharagpur

  111. Mr. A Mannan Choudhury, Consultant, Agri Livelihood, Dharapur, Guwahati, Assam

  112. Mr. A. Antony Prabaksingh, Terku Kallikulam – Tirunelveli district, Tamilnadu

  113. Mr. A.Periyakaruppan, Vilathur, Manamadurai, Sivaganga District, Tamilnadu

  114. Mr. Abdul Shukkoor PM, Mangad, Unnikulam, Kozhikode, Kerala

  115. Mr. Aditya Abhang, Software engineer, Hyderabad

  116. Mr. Ainthunai Velusamy, Ainthunai Natural farmers group, Erode, Tamil Nadu district

  117. Mr. Ajit Kumar Som,  Kolkata

  118. Mr. Akhilesh, Malappuram

  119. Mr. Ali Asghar Shabbir Ali, Architecture, Mumbai

  120. Mr. Amar Kumar Debnath, Awaken India Movement, Kalyani, West Bengal

  121. Mr. Amitabha Dev Choudhury, Retired Teacher, Silchar, Assam

  122. Mr. Anand Saksena, Retired, Jaipur

  123. Mr. Anandharasu, Business owner, Chennai

  124. Mr. Anantharaman V, Organic Farmer, Thiruvaiyaru

  125. Mr. Ananthoo, Safe Food Alliance, Chennai

  126. Mr. Anil Dev Verma, Farmer, Village Chandi PO Gondgiri District Bemetara, Chhattisgarh

  127. Mr. Animesh Kumar Chaudhary, Raipur, Chhattisgarh

  128. Mr. Anoopkumar AK, Agriculture, Kumbakonam

  129. Mr. Anshuman Das, Expert – Agroecology and Food Systems, Kolkata

  130. Mr. Anugrah Lakshmanan, Carnatic classical musician, Coimbatore

  131. Mr. Anvesh Reddy Sunketa, Telangana Kisan Congress, Hyderabad

  132. Mr. Arindam Bhattacharjee, Agriculturalist, Assam

  133. Mr. Arindam Tapaswi, Balurghat

  134. Mr. Arnab Chakraborty, Srijani Sanstha, Kolkata

  135. Mr. Arockia Prince, Agriculture, Tirunelveli, Tamilnadu

  136. Mr. Arpit Sharma, Farmer, Kota, Rajasthan

  137. Mr. Arul Jega Selvan, Farmer, Vadakankulam

  138. Mr. Arunkumar, Self employed, Kunnathurmedu, Palakkad, Kerala

  139. Mr. Arunprasath Venket Ramana Samy, Natural Farmer, Dharapuram

  140. Mr. Ashim Kumar Munshi, Retired, Nashik

  141. Mr. Ashis Biswas, Retired, Kolkata

  142. Mr. Ashish Arya, AIM, New Delhi

  143. Mr. Ashok Kumar Sehgal, Journalist, Ludhiana

  144. Mr. Ashok Raj, Farmer,

  145. Mr. Ashwin Purshottam Chavda, Motion Graphic Artist, Mumbai

  146. Mr. Asit Pal, Kolkata

  147. Mr. Asokakumar V, Kerala Jaiva Karshaka Samithi, Malappuram

  148. Mr. Athikesavan S, Software Engineer, Thanjavur

  149. Mr. Augustine Newton, Farmer, Kavalkinaru

  150. Mr. Avik Saha, Jai Kisan Andolan, Kolkata

  151. Mr. B R Narayanaswamy, Farmers’ Leader, Coimbatore

  152. Mr. Babu R, Farmer, Perambur village, Uthukottai taluk, Thiruvallur Dist., Tamilnadu

  153. Mr. Bala Dhandapani K, Independant Natural Farmer, Dharapuram Taluk, Tiruppur Dist, Tamil Nadu

  154. Mr. Balachandran C, Architect, Mysore

  155. Mr. Balaji Srinivasan, IT, Chengalpattu

  156. Mr. Balakrishnan, Farmer, Jawadhupatty

  157. Mr. Balamurugan, Engineering, Tirunelveli

  158. Mr. Balasubramanian, Farmer, Pudukkottai

  159. Mr. Baljinder Kamboj, Software engineer, Sirsa, Haryana

  160. Mr. Banbehari Ghar, Sonamukhi, Bankura, West Bengal

  161. Mr. Basavaraj Honnappa, Consultant for FPOs, Matala, Basavakalyan, Bidar, Karnataka

  162. Mr. Baskar Manimegalai, Nammalvar Multiversity, Tiruvannamalai

  163. Mr. Bhairab Saini, Panchal, Bankura

  164. Mr. Bharani, Dharapuram

  165. Mr. Bharat Dogra, Author, Delhi

  166. Mr. Bharath A C, Student, Bangalore

  167. Mr. Bhoopendra Singh, Sangata Purkhauti Agro Farmer’s Producer Company Limited, Ambikapur, District Surguja, Chhattisgarh

  168. Mr. Bijendra, Tutor, Haryana

  169. Mr. Biju Muttathusseril, Electrical, Thrissur

  170. Mr. Bishnu Rabidas,

  171. Mr. Biswajit Bhadra, West Bengal

  172. Mr. Bokul Chandra, Organic Farming, Thakurgaon

  173. Mr. Brijesh kumar, Account executive, Meerut

  174. Mr. Brindawan Das Mundhra, Retired, Bikaner, Rajasthan

  175. Mr. C Rajendran, Farmer, Erode

  176. Mr. Chandra, Bangalore, Karnataka

  177. Mr. Chandrasekaran Chinnasamy, Natural farming, Dharapuram

  178. Mr. Chandrasekaran, Tamil Nadu

  179. Mr. Chandrashekhar Hirachand Mendole, Assistant Professor, Buldhana Maharashtra

  180. Mr. Chellamuthu, Organic Farming and Engineering Professional, Coimbatore, Tamilnadu

  181. Mr. Chetan Singh Deora, General Manager, Jodhpur, Rajasthan

  182. Mr. Chidambaram AR, Agriculturist, Chennai

  183. Mr. Chirag Karania, Business owner, Mumbai

  184. Mr. Christopher Columbus, Engineer, Tamil Nadu

  185. Mr. Darshan P K, Purnapramati, Bengaluru

  186. Mr. Debashish Tarafdar, Kolkata

  187. Mr. Debjit Chakraborty, Art/Dance, Kolkata

  188. Mr. Deepak Malani, Agriculture allied, Ulhasnagar

  189. Mr. Deivasigamani, Art of Living, Tiruppur

  190. Mr. Devison A. K, General Secretary, Kerala Coconut Farmers Association, Kozhikode

  191. Mr. Dhamodharan, Organic shop, Medavakam

  192. Mr. Dharmendra Kumar, Jan Pahal, Delhi

  193. Mr. Dhivakar Prasanna Palanisamy, Agriculturist at Aervalam Organic Farm, Tamil Nadu

  194. Mr. Dhruv Gupta, Indian Seed Sovereignty Alliance, Powai, Mumbai

  195. Mr. Dibyendu Das, Junior Engineer, West Bengal

  196. Mr. Dilip Talreja, Retired, Mumbai

  197. Mr. Dinesh Kumar, Business, Chennai

  198. Mr. Dineshram KP, Nambiyur, Erode

  199. Mr. Divesh Trikha, Retired, Karnal, Haryana

  200. Mr. Divy Dholakiya, Business, Rajkot, Gujarat

  201. Mr. Doraisamy, Organic farmer, Thekkalur, Avinashi, Tiruppur

  202. Mr. Elango S, CA cum Natural Farmer, Annur, Coimbatore

  203. Mr. Esakkinatarajan, SSIASAT (Art of living) Bangalore, Palayapettai, Tirunelveli, Tamil nadu

  204. Mr. G Vijay, Raichur, Karnataka

  205. Mr. G. Subash, Software developer, Avinashi

  206. Mr. Gaindlal Chandrakar, Gau Sansad, Raipur

  207. Mr. Ganesh kumar, Buisness, Bangalore

  208. Mr. Ganesh Periyakaruppan, Chemical Engineer, Tamil Nadu

  209. Mr. Gautam Sonti, Filmmaker, Bangalore

  210. Mr. Gautham Ganesh PV, Law student, Lavad, Gujarat

  211. Mr. Geo Damin, Poovulagin Nanbargal, Chennai

  212. Mr. Girija Nandan Upadhyay, Ranchi

  213. Mr. Gopal Subhash Mahajan, Mumbai, Maharashtra

  214. Mr. Gopalakrishna Tikkireddy, BKS, Amalapuram, Andhra Pradesh

  215. Mr. Gopalakrishnan, Engineer, Mumbai

  216. Mr. Gopi, Safe Foods, Chennai

  217. Mr. Goutam Dass, Publishing Professional, Kolkata

  218. Mr. Govardhanan B, Vallalar Organic Herbals, Tiruvannamalai, Tamilnadu

  219. Mr. Govindaraj P, Software, Chennai

  220. Mr. Gowthamaraja Eswaramoorthy, IT, Dharapuram

  221. Mr. Gramiyam Palanisamy, Graamiya Saangamam, Tirupur

  222. Mr. Guramneet Singh Mangat, General Secretary, Progressive Farmers Front, Dal Nagar Farms, Sampurna Nagar, Tehsil Palia, Distt. Lakhimpur Kheri, UP

  223. Mr. Gurpreet Singh Sangha, Agriculture / International Trade, Jaipur

  224. Mr. Hariharan T S, Software engineer, Bangalore

  225. Mr. Hemadri Babu G, Ex Telecom Professional and now Active Natural Farmer, Tiruttani town, Tiruvallur district, Tamilnadu

  226. Mr. Hemendra Varma, Management Consultant, Pune

  227. Mr. Himakiran, Thondaimandalam Foundation, Komakkambedu, Tiruvallur, Tamil Nadu

  228. Mr. Himanshu Bhatt, IT Sector, Navi Mumbai

  229. Mr. Ilango, Farmer, Thalavadi

  230. Mr. Issac Devaraj, Traditional Rice Seller, Chennai

  231. Mr. J.Karuppasamy, Natural Farmer, Sivakasi, Virudhunagar Dist, Tamilnadu

  232. Mr. Jacob Sathiyaseelan, Organic farmer, Chennai, Tamilnadu

  233. Mr. Jaffar Sadiq, Social services, Trivandrum

  234. Mr. Janakiraman, IT, Porani, Karur

  235. Mr. Jawahar Subramanian, Agriculturist, Kondayampalayam, Kallippatti, Gobichettipalayam Taluk, Erode District, Tamilnadu

  236. Mr. Jayanta Bhattacharjee, Central Government Employee, Kolkata

  237. Mr. Jesuraj, Founder, Thaai Veedu, Tirunelveli

  238. Mr. Jet Lee Honhaga, Technician, Trichy

  239. Mr. Jeyakumar C, Acupuncture Therapist, Madurai

  240. Mr. Jitesh Jain, Student, Sawai Madhopur

  241. Mr. Joshi Deepak,    Gujarat

  242. Mr. Joydev Dey, Bijnan Darbar, Kanchrapara

  243. Mr. K R Dayananda, Retired Banker, Chennai

  244. Mr. K Ragupathy, Lawyer, Chennai

  245. Mr. K S Shimon, Mysore

  246. Mr. K. Balakrishnan, Samyukta Kisan Morcha (TN), Palavanthangal, Chennai

  247. Mr. K.Harish, Agriculturist, Nagercoil

  248. Mr. Kalimuthu K, Farmer, Dharapuram

  249. Mr. Kalyan Chakraborty, Business, Kolkata

  250. Mr. Kandavadivel P, Natural  farming, Avinashi

  251. Mr. Kannan Sethuraman, Gumalapuram

  252. Mr. Kapil Shah, Promotion of Organic Farming, Vadodara, Gujarat

  253. Mr. Karthik Gunasekar, Tamil Nadu Iyarkai Velan Koottamaipu, Chennai, Tamil Nadu

  254. Mr. Karthikeyan M, Yogacharya, therapist AYUSH certified, East Tambaram

  255. Mr. Karthikeyan Marimuthu, Farmer, Vadavedampatti, Sulur, Coimbatore

  256. Mr. Kaushik, Retired Electrical Engineer, Guwahati

  257. Mr. Keshab Kumar Ghosh, Natural Farming Trainer, West Bengal

  258. Mr. Kiran Rajendra Kamble, NGO worker, Pune

  259. Mr. Kishore Otturu, Engineer, Nellore

  260. Mr. Kishore Puvvada, Research Scholar, Independent Consultant, Sholinganallur, Chennai

  261. Mr. Kshitij Sharad Supekar, Vadodara

  262. Mr. Kuldeep Singh, Private employee, Delhi

  263. Mr. Kuldeepak B. Deshpande, Natural Farmer, Chhatrapati SambhajiNagar, Maharashtra

  264. Mr. Kumar S, Analyst, Srivilliputtur

  265. Mr. Kumar Velu, Radhapuram Taluk Farmers Association, Azhaganeri

  266. Mr. Kunal Brahmakshatriya, Accounts Manager, Ahmedabad

  267. Mr. Kunal Dutta, Teacher, West Bengal

  268. Mr. L. Pichandi, Farmer, Tamil Nadu

  269. Mr. Leonard,  Sirumalai Hills,  TN

  270. Mr. Logesh V, Farmer and photographer, Bhavani, Erode, Tamilnadu

  271. Mr. M Karthick Abhiraj, ABHI in Naturals, Madurai

  272. Mr. M N Dinesh Kumar, Earth360 Eco Ventures Private Limited, Kadiri, Andhra Pradesh

  273. Mr. M. Karthickumar, Vanam India Foundation

  274. Mr. M. Meganatha Sivasankaran, Journalist, Vettavalam, Tamilnadu

  275. Mr. Madan Lal, Software professional, Bangalore

  276. Mr. Madhanagopal, Manager, Madurai

  277. Mr. Mahaveer Singh, Social Worker, Bareilly, Uttar Pradesh

  278. Mr. Mahesh Bhatt, Retired Assistant Professor. Bhavnagar, Gujarat

  279. Mr. Manasha Chandra Maharana, NIRMAN, Odisha,

  280. Mr. Maneesh Yadav, Banker, Gurugram

  281. Mr. Manickavasagam, Banker, Madurai

  282. Mr. Maninder Kumar, Farmer, Mohali

  283. Mr. Manoj, Farmer, Surat

  284. Mr. Manoranjan Swain, Odisha Right to Food, Bhubaneswar

  285. Mr. Mantu Kumar Sau,    Purba medinipur

  286. Mr. Mariappan Krishnasamy, Engineer, Town

  287. Mr. Marutham Kumar, Agriculturist, Gobichettipalayam

  288. Mr. Mary Jenit, Engineer, Chennai

  289. Mr. Mehul Raval, Trader, Gandhinagar, Gujarat

  290. Mr. Michael de Magry, Electrical, Bangalore

  291. Mr. Mohammed Rizwan.A., Acupuncturist, Tamil Nadu

  292. Mr. Mohan Dhayalan, Farmer, Kallakurichi,  TN

  293. Mr. Monisaanth T R, Farming, Kovilpalayam, Tiruppur, Tamilnadu

  294. Mr. Monuhar Pegu,    Agaratoli, Bokakhat, Kaziranga, Assam

  295. Mr. Mukesh Lutade, Magan Khadi, Wardha

  296. Mr. Mukesh Marhia, Malad West Mumbai Maharashtra

  297. Mr. Munish Kumar, Climate Solutions

  298. Mr. Muralitharan, Farmer, Tiruppur, Tamilnadu

  299. Mr. Muthusamy K, Farmer

  300. Mr. Muthusamy Srinivasan, Garments, Punjai Puliampatti, TN

  301. Mr. Nagabhushan, Manager, Bengaluru, Karnataka

  302. Mr. Nagarajan Mani, Karima Organic Farmers Market, Chennai

  303. Mr. Nagoormeeran K, Natural Farmer, Madurai, Tamilnadu

  304. Mr. Naresh Sharma, Eco Friendz, Jalandhar

  305. Mr. Natarajavel B, Tamil Nadu

  306. Mr. NatrajKumar Chandrasekhar, Senior IT leader, Chennai

  307. Mr. Neetesh Jain, Farmer, Damoh, Madhya Pradesh

  308. Mr. Nidhin Jacob, IT Professional, Kochi, Kerala

  309. Mr. Nighil K, Punalur

  310. Mr. Nirmalendu Jyotishi, Development Consultant, Bhubaneswar, Odisha

  311. Mr. Nitin Sali, V R S, Pune

  312. Mr. Nitin Saxena, Pilot, Mumbai

  313. Mr. Nitinn Relekar, Photographer, Navi Mumbai

  314. Mr. P Govindasamy, Retired, Tamil Nadu

  315. Mr. P K Hariharan, Bangalore

  316. Mr. P R Rangaswami, Aram Organic Farmers Producer Company Ltd., Coimbatore

  317. Mr. P Srinivas, SOIL, Bengaluru

  318. Mr. Padam Kumar Jain, Prem Samriddhi Foundation, Bundi

  319. Mr. Pandiaraj, Engineer, Tamil Nadu

  320. Mr. Paneer Selvam, Farmer, Tamil Nadu

  321. Mr. Pankaj Malviya, Engineer, Member of IEEE, Bengaluru

  322. Mr. Parag R, Teacher, Chennai

  323. Mr. Paranthman.M, Assistant Director, Chennai

  324. Mr. Pareshkumar Dave, Retired, Anand

  325. Mr. Partha Mazumder, West Bengal

  326. Mr. Partha Sarathi Mallick, Teacher, Sonarpur, West bengal

  327. Mr. Parthasarathy VM, Farmer, Pandeswaram,  Thiruvallur, Tamil Nadu

  328. Mr. Parthiban, Agriculturist, Cuddalore

  329. Mr. Parthiban.C, Transee Organics, Chennai

  330. Mr. Pasumai Desam Rajendar, Pasumai Desam, Coimbatore

  331. Mr. Periyasamy Sennimalai, Retired, Kannamanaicknur

  332. Mr. Philip Martis, Holistic Healer, Pune, Maharashtra

  333. Mr. Piru Pal, Krishan, West Bengal

  334. Mr. Pothi, Freelancer, Chatrapatti, Viruthunagar District, Tamilnadu

  335. Mr. PP. Shankaran, Salem

  336. Mr. Prabakaran, Kumbakonam

  337. Mr. Prabhakaran Apparsamy, Traditional Organic Farming, Avinashi

  338. Mr. Prabir Banerjee, Environmental work, Pondicherry

  339. Mr. Pradeep Golecha, Business, Rajasthan

  340. Mr. Pradeep Kumar Dhandapani, Bengaluru

  341. Mr. Prajwal, Mysuru University, Karnataka

  342. Mr. Prakash Udikeri, IT Sector, Belgaum, Karnataka

  343. Mr. Prameet, Yoga teacher, Mumbai

  344. Mr. Prasad Suryawanshi, Mechanical Engineer, Mumbai

  345. Mr. Prashanth Bachu, Urban Transport Specialist, Hyderabad

  346. Mr. Prashanth Jayaram, Farmer, Sathegala village, Karnataka

  347. Mr. Prateek Das, Airline Pilot, Kolkata

  348. Mr. Pratham  Jain, Businessman, Chennai

  349. Mr. Pratikbhai Narendra bhai Barbhaya, Stationery vendor, Ahmedabad, Gujarat

  350. Mr. Praveen Kumar AR, Agriculture, Hyderabad, Telangana

  351. Mr. Praveenkumar, Agriculture, Tamil Nadu

  352. Mr. Preamkumar,    Trichy

  353. Mr. Pritam Bhowmick, Chemist, West Bengal

  354. Mr. Priyabrata Panja, Buiness, West Bengal

  355. Mr. Puneet Arora, Business/Pharmacist, Delhi

  356. Mr. Puran Bartwal, People’s Science Institute

  357. Mr. Purushothaman Balakrishnan, Software, Bangalore, Karnataka

  358. Mr. R Rajesh Kumar, Venmathis Nature, Perumampalayam, Samalapuram, Tirupur, Tamilnadu

  359. Mr. Rafikulalam Sahana, Farmer, Bardhaman, West Bengal

  360. Mr. Raghunathasethupathy, Software Engineer, Tamil Nadu

  361. Mr. Raj Kamal, Teacher, Haridwar

  362. Mr. Raja Paul, Farmer, Vadakangulam, Tirunelveli, Tamil nadu

  363. Mr. Raja Sankar, Agripreneur – Purva Naturals, Coimbatore, Tamil Nadu

  364. Mr. Rajamani Krishna Kumar, Co ordinator EDOFF, Gobichettipalayam,  Tamilnadu

  365. Mr. Rajasekar Krishnamurti, President, Tamil Nadu

  366. Mr. Rajesh Shervegar, Business, Udupi

  367. Mr. Rajeshkannan,    Kovilpatti

  368. Mr. Rajneesh Bedi, Agriculturist, Bugga Kalan

  369. Mr. Rakesh Kumar Singh, Farmer, Sheohar, Bihar

  370. Mr. Rama rao jonnalagadda, Farmer, Guntur

  371. Mr. Ramakrishna Naidu E, Sundaramma Mango Farms, Ramapuram

  372. Mr. Ramakrishnan Manden Kattil, Audio engineer, Mumbai, Maharashtra

  373. Mr. Ramasamy Selvam, EDOFF, Erode, Tamilnadu

  374. Mr. Ramasubramanian, Senior Sustainability Specialist / Director, Samanvaya Social Ventures, Tamil Nadu

  375. Mr. Ramesh Chandran, Founder, CEO of Bio Basics, Coimbatore

  376. Mr. Ramesh N, Agriculturist & Engineer, Dharapuram

  377. Mr. Ramesh Ramasamy, Agriculture, Mulanur

  378. Mr. Ramesh, Agriculture, Kovilpatti

  379. Mr. Ramkumar, Tamil Nadu

  380. Mr. Ramnarayan K, Natural History Educator, Munsiari, Uttarakhand

  381. Mr. Ramprasad Sana, Agricultural Scientist, Hyderabad

  382. Mr. Ranganathan, Farmer, Dharapuram

  383. Mr. Raspinder Singh Grewal, Natural farming, Village Sherpur kalan, Punjab

  384. Mr. Rathnakumar, Engineer, Ramapuram

  385. Mr. Ravi Kumar Singh, Agribusiness Entrepreneur, Rajkheta, Wadrafnagar, District- Balrampur, Chhattisgarh

  386. Mr. Ravi Shankar, CBIC officer, Delhi

  387. Mr. Ravi SP, Chalakudypuzha Samrakshana Samithi, Chalakudy, Thrissur District, Kerala

  388. Mr. Ravi.K., Farmer, Tamil Nadu

  389. Mr. Riddhiman Basu, IT Professional, Kolkata

  390. Mr. Rohan Patil, Software consultant, Mumbai

  391. Mr. Rohan Vakharia, Maharashtra

  392. Mr. Ruhant Kini, Trader, Mumbai

  393. Mr. Rushil Tamboli, Awaken India Movement, Bhavnagar, Gujarat

  394. Mr. S Syed Noor Mohamed, Farmer, Tamil Nadu

  395. Mr. S.Thirunavukkarasu, Retired, Chennai

  396. Mr. Sabari Karthik, Microbiologist, Coimbatore

  397. Mr. Sachin Kulkarni,  Shelgaon, Jalna

  398. Mr. Saikat Sengupta, Software Engineer, Kolkata

  399. Mr. Sajan, Chennai

  400. Mr. Sanjay Dixit, Farmer leader, Agra, Uttar Pradesh

  401. Mr. Sanjay Kanti Chanda, Busineas Consultant, Bengaluru

  402. Mr. Sanjay Kumar, Society For Promotion of Wastelands Development, West Bengal

  403. Mr. Sanjay Lakra, Social service centre, Jharsuguda, Orissa

  404. Mr. Sanjay Rajesh Gupta, Advocate, Mumbai

  405. Mr. Sanjeev Kumar Singhal, Farmer, Bulandshahar

  406. Mr. Sanjib Kumar, Lawyer, Odisha

  407. Mr. Sanjoy Das, Technician, West Bengal

  408. Mr. Sankar A., Farmer, Bellepalayam Village, Tamil Nadu

  409. Mr. Saravanan Durai, Engineering, Chennai

  410. Mr. Saravanan, Athinathatraders,  Tamil Nadu

  411. Mr. Sathees Kumar B, Kerala  Jaiva Karshaka Samithi, Thuravoor P O, Alappuzha, Kerala

  412. Mr. Sathishkumar, Engineer, Gobichettipalayam

  413. Mr. Satish Padyal, Office Assistant, Mumbai

  414. Mr. Satya, IT, Bengaluru

  415. Mr. Saurabh Sahni, Namma Farmers Market, Mysore

  416. Mr. SB N Quadri, Lawyer, Aurangabad

  417. Mr. Selvakumar, Natural Farmer, Theni

  418. Mr. Selvam Ramasamy, Tamilnadu Organic Farmers Federation, Tamilnadu

  419. Mr. Senthilkumar Thirumalaisamy, Agriculture, Coimbatore

  420. Mr. Senthilkumar, Agricultural, Tiruppur

  421. Mr. Shanmuga Subramanian Vellagal Arunachalam, Farmer, Chennai

  422. Mr. Sheik Abdul Safur, Engineer, Tamilnadu

  423. Mr. Shiburaj AK, Journalist, Kozhikode, Kerala

  424. Mr. Shijil, Ottapalam, Palakkad

  425. Mr. Shiva Ganesh, Farmer, Thatchur, Arani

  426. Mr. Shivansh Vishwakarma, Self Employed, Thane

  427. Mr. Shoaib Chadkhan, Entrepreneur, Mysore

  428. Mr. Shridhar Shankar Mahajan, Pune, Maharashtra

  429. Mr. Shrikikrishna Joshi, Interior designer, Pune, Maharashtra

  430. Mr. Shubham Jade, Student, Mumbai

  431. Mr. Shyam, Banker, Chennai

  432. Mr. Siddharth Singh Negi, Uttaranchal Youth and Rural Development Centre, Narayanbagar, District Chamoli,  Uttarakhand

  433. Mr. Sivabalan Athilingam, Chennai

  434. Mr. Somenath Dey, Telecom engineer, West Bengal

  435. Mr. Somnath Chakraborti, Assistant Professor, Halisahar

  436. Mr. Somnath Sil,    Siliguri

  437. Mr. Somu, LLP, Mylapore, Chennai

  438. Mr. Soumik, Bharat Beej Swaraj Manch, Balaghat, Madhya Pradesh

  439. Mr. Soumyendra Roy, Consultant, Kolkata

  440. Mr. Sourabh Kumar Roy,    Coochbehar

  441. Mr. Sourav Ghosh, West Bengal

  442. Mr. Sourav Mukherjee, IT professional, New Delhi

  443. Mr. Souvratra Chakraborty, Business, Kolkata

  444. Mr. Sridharan K, Media, Thanjavur

  445. Mr. Subir Majumdar, Musician, Kolkata

  446. Mr. Subramanian P N, Regenerative natural farmer, Padur, Tamilnadu

  447. Mr. Sudhagar, Auto driver, Uthukkadu

  448. Mr. Sudhakar, Kannamangam

  449. Mr. Sudip Ghosh, Teacher, Baharampur, West bengal

  450. Mr. Sudipta Jana, Farmer, Village Bilara PO Hawakhana, West Bengal

  451. Mr. Sujanand P, Vellore

  452. Mr. Sukanta Chakraborty, Business owner, Durgapur, West Bengal

  453. Mr. Sukanta Mondal, Kolkata

  454. Mr. Sukumaran M, Agriculture, Kuttiattoor

  455. Mr. Sundara Vimalanathan, Tamil Nadu Cauvery Farmers Protection Association, Swamimalai PO, Thanjavur district, Tamil Nadu

  456. Mr. Sundararajan, Pollachi

  457. Mr. Sundaresan Kaliyaperumal, Organic Farmer, Jayankondam, Ariyalur, Tamilnadu

  458. Mr. Sunil Bavkar, IT, Navi Mumbai

  459. Mr. Sunny Jain, Surat, Gujarat

  460. Mr. Suresh Kanna, BERAS India, Trichy

  461. Mr. Suryanarayana Tummala, Farmer, Chennai Tamilnadu

  462. Mr. Sushant Maurya, Farmer, UP

  463. Mr. Suvendu Singha, Training Expert, Ranchi

  464. Mr. T.R Santhoshkumar, Farmer, Palakkad

  465. Mr. Tadepalli Krishnaprasad, Certified Clinical Nutritionist, Tupran, Telangana

  466. Mr. Tamilarasan, Farmer, Tiruppur, Tamilnadu

  467. Mr. TANMOY MAHATO, Student, Jharkhand

  468. Mr. Tapas Biswas, Paribesh Bandhab Mancha Barrackpore, West Bengal

  469. Mr. Tejas D Vora,Chartered Accountant, Gujarat

  470. Mr. Thilak Raj S, Safe Food Alliance, Chennai

  471. Mr. Thirumurugan, Farmer, Chidambaram Tamilnadu

  472. Mr. Tulsidas Kamath, Mechanic, Kumta

  473. Mr. Uday Chandra Ghosh,    West Bengal

  474. Mr. Uthayakumar R, Agriculture, Tamil nadu

  475. Mr. Utkarsh Tawade, Port Authority worker, Navi Mumbai

  476. Mr. V Ramasamy, Auditor, Tirupur

  477. Mr. V. Suresh Babu, Chennai

  478. Mr. V.Goverthan, Farmer, Kettavaram palayam, kalasapakkam, Tiruvannamalai , Tamilnadu

  479. Mr. Vaidyanathan, Velachery

  480. Mr. Vansh Narang, Research Psychologist, Delhi

  481. Mr. Veeraragavaprabu Ponraj, Software Programmer, Chennai

  482. Mr. Velmurugan, Material specialist, Tamil Nadu

  483. Mr. Venkatesh, Farmer, Raichur, Karnataka

  484. Mr. Venugopal, Business, Punjai Puliampatti

  485. Mr. Venugopalan Govindan, Qualitime Software Private Limited, Coimbatore

  486. Mr. Vijay Krishna Raichurkar, IT professional, Pune

  487. Mr. Vijayakanth Borus, Farmer, Anaigudi, Tirunelveli, Tamilnadu

  488. Mr. Vikram Kumaraswamy, IT services, Bangalore

  489. Mr. Vilas Pralhad Sheware, Ashirwad foundation, Nagpur, Maharashtra

  490. Mr. Vinay Verma,    Pune, Maharashtra

  491. Mr. Vineet Pandey, Teacher, Mathura

  492. Mr. Virendra Parekh, Journalist, Mumbai

  493. Mr. Virupaxgouda Patil, Agriculture, Kurtakoti

  494. Mr. Vishal Lalka, Chartered Accountant, Gandhidham

  495. Mr. Vivek Kala, Engineer, Rajasthan

  496. Mr. Vivek Subramani, Kodaikanal

  497. Mr. Waseem Akram, Electrical Supervisor, Ramnagar, Bihar

  498. Mr. Yalalram Saw, Raigarh, Chhattisgarh

  499. Mr. Yash Agrawal, Assistant Professor of Philosophy, Navi Mumbai, Maharashtra

  500. Mr. Yash Saxena, Food researcher, Kargil

  501. Mr. Yashas M, Engineer, Mysore city, Karnataka

  502. Mr Yudhvir Singh, Bharatiya Kisan Union (BKU Tikait), New Delhi

  503. Mr. அருள் CM (Arul), Farmer, Avinashi

  504. Mrs. Bhavreen Kandhari, Clean Air Strategist, New Delhi

  505. Mrs. Bina Nissar, Mumbai, Maharashtra

  506. Mrs. Brigitte Maria Louis Zacharia, Farmer, Mysore

  507. Mrs. Chandiralekha, IT, Chennai, Tamil Nadu

  508. Mrs. Chandrika, Retired, Chennai, Tamilnadu

  509. Mrs. Chetana K, Farmer, Mumbai

  510. Mrs. Dhivya, Dindigul

  511. Mrs. Durgalakshmi V,  Chennai

  512. Mrs. G A S, Ahmedabad

  513. Mrs. Gayathree Krishna, CORI Engineers Pvt. Ltd., Chennai

  514. Mrs. Gayathri R, Chennai, Tamilnadu

  515. Mrs. Geeta Chandak, Software Consultant, Chhindwara

  516. Mrs. Geeta N Soneji, Hyderabad

  517. Mrs. Geetha S, Consultant, Tambaram

  518. Mrs. Geetha Sadasivam, Retired bank official, Thiruvanmiyur, Chennai

  519. Mrs. Geetha, Chennai

  520. Mrs. Gita Ram, Chennai

  521. Mrs. Gomathi, Tirunelveli

  522. Mrs. Gowsalya Palanisamy, Tamil Nadu

  523. Mrs. Grace Anitha, Kalanjiam Pengal vivasaya sangam, Chengam taluka, Thiruvannamalai, Tamilnadu

  524. Mrs. Ila Vaidya, Mumbai

  525. Mrs. Isha Deshpande,    Maharashtra

  526. Mrs. Jahnavi, TULA, Hyderabad

  527. Mrs. Jayashree Narayanan, Bharathanatyam Guru, Pondicherry

  528. Mrs. Jenny, Environmental Engineer, Poovulagin Nanbargal, Chennai

  529. Mrs. Jhansirani Otturu, Nellore

  530. Mrs. Jyothi Gokul, Natural farming – individual undertaking (moved from inorganic to natural farming processes), Chennai, Tamil Nadu

  531. Mrs. Jyothi, Yoga therapist, Chennai

  532. Mrs. Kala Venkteshwar, Director Private limited company, Chennai

  533. Mrs. Kiran Philip Martis, Awaken India Movement, Pune, Maharashtra

  534. Mrs. Kirti Mangrulkar, Farmer and entrepreneur, Nagpur

  535. Mrs. Kirti Negi Bajoria, Eco Warrior Enterprises, Chennai, Tamil Nadu

  536. Mrs. L. Uthira, Associate Professor in Nutrition, Coimbatore, Tamilnadu

  537. Mrs. Latha Palanisamy, Teacher, Kannamangalam

  538. Mrs. Lina Das De, Supervisor,

  539. Mrs. Lolita Ghosal, Associate Professor, Bally, Howrah, West Bengal

  540. Mrs. Madhura Shrinivas Gokhale, Natural farming, Pune, Maharashtra

  541. Mrs. Madhuri Sarang Inamdar, Advocate, Pune

  542. Mrs. Mahimkar Utsavi, Pharmacist, Mumbai

  543. Mrs. Malathi.K, Chennai

  544. Mrs. Manasa Veena, Teacher, Hyderabad

  545. Mrs. Manjushree Tadvalkar, Know How Foundation- INORA, Pune

  546. Mrs. Meera Korwar, Retired Banker, Mysuru

  547. Mrs. Mekala Jagadeeswaran, Tamilnadu

  548. Mrs. N.Manikavalli, Tirupur

  549. Mrs. Neeta B,    Pune, Maharashtra

  550. Mrs. Neeta Chandrashekhar Joshi,    Pune

  551. Mrs. Nilima Bhure,    Pune Maharashtra

  552. Mrs. Nisha Koiri, Awaken India Movement, Mumbai

  553. Mrs. Nitya Shaghana Selvaraj, Private sector, Trichy

  554. Mrs. Pallavi Singhal, Teacher, Mumbai

  555. Mrs. Pallavi Sulgekar,    Pune

  556. Mrs. Paramjit Kaur Narang, Sugam NGO, Gurugram, Haryana

  557. Mrs. Parveen kaur,    Pune

  558. Mrs. Pratibha Bharadwaj, Teaching profession, Jaipur, Rajasthan

  559. Mrs. Pratibha Nair,    Pune, Maharashtra

  560. Mrs. Purva Deepak Atre.,    Pune, Maharashtra

  561. Mrs. Rajalakshmi, Tamil Nadu

  562. Mrs. Rajani Kariappa, Bangalore

  563. Mrs. Rajeswari, Agriculture, Dharapuram

  564. Mrs. Rajisriram, Tirupur, Tamilnadu

  565. Mrs. Ramyachithra. K. R, Agriculture, Vellangallur

  566. Mrs. Rega Selvakumar, Profession, Tamilnadu

  567. Mrs. Renuka K.Narasimhan, Homemaker, Chennai, Tamil Nadu

  568. Mrs. Reshmi Hela Debnath,    Kolkata

  569. Mrs. Rucha Vikrant Patwardhan, Training, Pune

  570. Mrs. S Bharadwaj, Responsible Citizen, Chennai Tamil Nadu

  571. Mrs. S.Lilly Gracy, Teacher, Veerapandianpatnam,Tuticorin, Tamilnadu

  572. Mrs. Sagayaselvi, Chennai

  573. Mrs. Sameera, Marketing and Communications, Hyderabad

  574. Mrs. Samyuktha J, Farmer, Coimbatore

  575. Mrs. Sangeetha, Tirupur, TN

  576. Mrs. Sarika Santosh Rasane,    Pune

  577. Mrs. Sathya Senthilkumar, Chennai

  578. Mrs. Savitha Kamath, Maharashtra

  579. Mrs. Seethalakshmi, Chennai

  580. Mrs. Shabnam Narula, Bhoomi, Delhi NCR

  581. Mrs. Shanthi Krishnasamy, Sembulam organic farmers, Uthupalayam Dharapuram

  582. Mrs. Sheela Swaminathan, Bengaluru, Karnataka

  583. Mrs. Shivashanthalatha,    Perumbakkam, Chennai

  584. Mrs. Shoba Karuppiah, Chennai

  585. Mrs. Sivasankari  Venkatasubramanian, OFM, Chennai

  586. Mrs. Sreedevi L, Social Entrepreneur, Coimbatore

  587. Mrs. Sreeja K V, Patasala, Arangottukara, Arangottukara, Thrissur Kerala

  588. Mrs. Sreelatha Rajan, Farmer

  589. Mrs. Subha, Chennai, Tamilnadu

  590. Mrs. Subhashini, Chennai

  591. Mrs. Suguna, Advocate, Tirupur

  592. Mrs. Sumaiya Parveen, Assistant Manager, Chennai

  593. Mrs. Suparna Mukherjee, Teacher, Birbhum, West Bengal

  594. Mrs. Surya Devi M S, Acupuncturist, Coimbatore, Tamil Nadu

  595. Mrs. Swaroopa N B, Bangalore

  596. Mrs. Syamala Devi, Farmer, Thummalapalli, Vikarabad Dist, Telangana

  597. Mrs. Tithi Halder, Assistant Teacher, Chinsurah

  598. Mrs. Uma Maheswari RG, Information Technology, Tiruppur, TamilNadu

  599. Mrs. Usha Hariharan, reStore, Bengaluru

  600. Mrs. Ushajanaka, Retired, Chennai

  601. Mrs. Vanathi Sethuraman, Chennai, Tamil nadu

  602. Mrs. Varsha Mam, Photographer, Mumbai

  603. Mrs. Vasudha Deshpande, Rtd., Pune

  604. Mrs. Veena M, Freelance Environment Educator, Trivandrum

  605. Mrs. Vijaylakshmi Joshi, PUCL, Jaipur, Rajasthan

  606. Mrs. Vithya, Tamil Nadu

  607. Mrs. Vrushali Pandhare,    Pune, Maharashtra

  608. Mrs. Yamuna, Tamil Nadu    

  609. Mrs. Yeshmik Ponnappa, Director, Sapient College, Mysore, Karnataka

  610. Ms. Abhaya G, Corporate Lawyer, Mumbai

  611. Ms. Abhipsa Routray, Marketing executive, Bhubaneswar, Odisha

  612. Ms. Aditi Jain, Textile Designer, Chennai

  613. Ms. Ahana Rao, Architect, Goa

  614. Ms. Aishvarya Ramasami, Chennai

  615. Ms. Amardeep Kaur, Teacher, New Delhi

  616. Ms. Ameeta Shah, Psychotherapist & wellness coach, Mumbai, Maharashtra

  617. Ms. Anagha BK, Business owner, Pune, Maharashtra

  618. Ms. Anagha Walvekar, Technical writer, Pune, Maharashtra

  619. Ms. Anita Gandhi, Teacher, Kandivali, W Mumbai

  620. Ms. Anitha babu, IT, TamilNadu

  621. Ms. Anupama Madan, Yoga instructor, Mysore

  622. Ms. Anushka Kale, Independent, Pune, Maharashtra

  623. Ms. Aparna Bangia, Co founder of Earth4ever, Mumbai / Wada

  624. Ms. Aruna Rodrigues, Environment/Agri Lead Petitioner SC PIL for Moratorium on GMOs, Madhya Pradesh

  625. Ms. Atreyee Day, Artist Writer Facilitator, Calcutta

  626. Ms. Brindha Sriram, Chennai

  627. Ms. Chanda Asani, Tiruchirapalli

  628. Ms. Chenddyna Schae, Cafe owner, Bangalore

  629. Ms. Chithra Viswanathan, Teacher, Chennai

  630. Ms. Dharmambal N A, Chartered Accountant, Ayalur

  631. Ms. Diksha Bhosale, Student, Mumbai

  632. Ms. Divya, Chennai

  633. Ms. G.Kavya, Tutor, Chennai

  634. Ms. Gowri Seeragam, Native Store, Coimbatore

  635. Ms. Harpreet Singh, Entrepreneur and executive, Mysore, Karnataka

  636. Ms. Hetal, Farmer, Bharuch

  637. Ms. K Uma, Water management consultant, Bangalore

  638. Ms. Kajori Aikat, Kolkata

  639. Ms. Kalpana Manohar Dumale, Mumbai

  640. Ms. Karthika J, Chartered Accountant, Bangalore

  641. Ms. Kavya Chowdhry, Independent Researcher, Bangalore, Karnataka

  642. Ms. Krithika, IT, Coimbatore

  643. Ms. L Keshavamurthy, Farming, Hosur

  644. Ms. Latha B, Independent, Bengaluru

  645. Ms. Mahrukh Bulsara, Self employed, Mumbai

  646. Ms. Mallika Wwaminadhan, Chennai

  647. Ms. Manasi Baindur, Operations Executive, Vadodara, Gujarat

  648. Ms. Meera Goradia, Craft Sector, Mumbai

  649. Ms. Meera Sanghamitra,    Hyderabad

  650. Ms. Nalini Nair, Working with rural women, Trivandrum

  651. Ms. Namrata Bhutoria, Natural Dye educator, Mysore

  652. Ms. Narasimha Kamath, Mechanic, Kumta

  653. Ms. Natasha Agrawal, meditation healing, Mumbai

  654. Ms. Neesha Noronha, Freelance facilitator, Mysore

  655. Ms. Neetha Narresh, Eco- Volunteer, Chennai

  656. Ms. Nida Fatima A B, ISL Interpreter, Mysore

  657. Ms. Nirali Vaidya, Writer, Mumbai

  658. Ms. Nisha Talreja, Yoga instructor, Mumbai

  659. Ms. Nivedita R, TNIVK, Chennai

  660. Ms. Pavithra A, Farming, Kerala

  661. Ms. Payal Jain, Senior Compliance Manager, Bangalore

  662. Ms. Pochi S, Nutrition, Nashik

  663. Ms. Prabhs,    Uttrakhand

  664. Ms. Pranitha, Social worker, Chennai

  665. Ms. Priyanka Mohan, Environmentalist, Chennai

  666. Ms. Purobi Menon, Language Instructor, Uttar Pradesh

  667. Ms. Purwa Kushwaha, Freelance translator, Hyderabad

  668. Ms. Radhika Rammohan, Director, Restore, Health, Livelihoods and Nature, Chennai

  669. Ms. Ramba B, Powergrid

  670. Ms. Rashmi Mahajan, Researcher, Delhi

  671. Ms. Reema Shah, Freelancer, Mumbai, Maharashtra

  672. Ms. Rosamma Thomas, Journalist, Pala town, Kerala

  673. Ms. Samita Mangat    

  674. Ms. Seema Gupta, Communication Facilitator, Surat, Gujarat

  675. Ms. Sejal Dand, MAKAAM, Ahmedabad

  676. Ms. Shaikh Zoha, Student, Mumbai

  677. Ms. Sharmita Ghosh, Research scholar, Navi Mumbai

  678. Ms. Shashirekha.M.G, Principal, Purnapramati, Bengaluru

  679. Ms. Sheela Balaji, Social entrepreneur and organic farmer, Chennai

  680. Ms. Sheelu Francis, Women’s Collective, Chennai

  681. Ms. Shweta Bhattad, Gram Art Project, Village Paradsinga, Tehsil Sausar, District Chhindwara, Madhya Pradesh

  682. Ms. Subha Bharadwaj, Safe Food Alliance, Nava Raipur, Chhattisgarh

  683. Ms. Sudha Gandekar, NGO, Chennai

  684. Ms. Sudha N, Independent researcher-activist, Bangalore, Karnataka

  685. Ms. Suma Josson, Film maker, Nagpur, Maharashtra

  686. Ms. Sushmitha R.K., Fashion designer, Karnataka

  687. Ms. Tara Aslam, Sustainable living, Bangalore

  688. Ms. Tejal, Freelance Facilitator, Mumbai

  689. Ms. Ulka Shukla, Navi Mumbai

  690. Ms. Upma Sharma, Photography, Jhunjhunu

  691. Ms. Usha Kumari, Agroecology educator, Trivandrum

  692. Ms. Utkarsha Shelke, Pune

Annexure:

There is inadequate information on the impact of on-target and off-target mutations that happen in gene edited crops, making them a risky enterprise

Gene Editing cannot be equated with Natural Mutations as they are not random and it affects all the copies of the gene in the plant as well as regions that are protected and conserved naturally. There could be foreign/exogenous genetic material embedded in the new mutants in the process of transformation and thus, these varieties could be Transgenics. Antibiotic Marker genes have been used for creating these mutants and they might be transmitted to other organisms through Horizontal Gene Transfer.

SDN-1 Gene Editing may lead to unintended off target mutations. Due to random repair mechanisms, it may also lead to structural changes – translocations, duplications, inversions of gene segments. Due to random repair of spliced genes, there could be epigenetic changes. This can generate traits with unknown impacts and lead to toxicity of the environment or impact human & animal health. Such mutant plants cannot be equated with their parents or conventionally-bred plants as the genetic makeup has changed and it is not clear how this new genetic change will react with the environment. Gene Edited Plants can contaminate other conventional varieties & wild relatives. 

The two mutated genes in the two rice varieties developed in India have several known functions including uptake and regulation of nutrients. The long term impact of such changes are not clear without enough experiments and safety research. This may lead to Chromothripsis -where several Genes are changed simultaneously affecting the whole genome.

In the USA, the two cultivated gene-edited maize varieties are for traits that have been earlier created through transgenic technology – insect resistance and herbicide tolerance! What’s more, both these varieties are also transgenic. It is clear where the so-called latest cutting edge technology is actually leading towards. Meanwhile, since there are only a handful of Gene Edited crops being newly developed (Rice, Soybeans, Tomato, Banana, Maize, Cacoa, Pepper, Potato, Wheat, Alfalfa & Flax), evidence on the negative impacts are not yet available widely.

Differences between conventional breeding (including conventional mutation-breeding) and Gene Editing:

Conventional Breeding, including induced mutations (conventional mutation breeding through irradiation/chemicals)

Genome Editing

Random – leading to a range of variations that would then be naturally selected.

Largely specific in terms of geneticist’s desired change. However, many random changes do occur here

Takes up the whole plant or cell, and does not directly interfere at the DNA level

Directly interferes with genetic materials – DNA, RNA, proteins

Mutation results depend on several factors, many of which are natural

Bypasses natural gene regulation almost completely

No foreign genes involved specifically in the process of breeding, except where crossing across species is attempted

Foreign genes are involved – several exogenous genetic materials are used in the vector. In some gene editing applications, a foreign gene is sought to be inserted in the target site. Even in the former case (materials in the vector), unintended insertions of foreign genetic material have been reported

Antibiotic-Resistant Marker genes are not involved 

Antibiotic-Resistant Marker genes are used

These breeding methods do not change all genetic sequences at the same time

Similar gene clusters are all changed at the same time, genome-wide

Several gene segments are protected and conserved, and largely remain unaffected, the way natural evolution has meant it to be

Protected gene areas can also be changed

Original gene segments are still maintained and can serve as templates for repair

All the targeted gene copies are changed. The change is irreversible thereafter

Changes are not specific for each method

Can change several of the same, or different genes at the same time leading to substantial changes in the biological characteristics of the plant

INDEL analysis shows lesser number of changes occur in conventional mutation-breeding, with larger random changes

There are more changes that occur here, but of shorter size/length.

It is clear that genome editing cannot be equated with conventional mutation-breeding, as is being done by the Government of India at this point of time. 

Scientific Evidence that shows the need for regulating in a comprehensive and independent fashion SDN-1 and SDN-2 technologies also, as part of regulating all genome editing

Prior to the announcement of the gene-edited rice varieties and development of gene-edited sheep, the Government of India has for all practical purposes deregulated two gene-editing technologies,  SDN-1 and SDN-2. In the past, the Coalition had already written (here and here) to various Government authorities and departments pointing out the dangers of de-regulating gene-editing technologies, pointing out the irreversible risks associated with it. The Coalition had also given detailed feedback on strengthening the regulation for gene-edited crops in India.

Gene Editing is Genetic Engineering as per EPA’s 1989 Rules

Gene Editing is also genetic modification as is apparent from the statutory definition in India of Genetic Engineering. Environment Protection Act 1986’s relevant statutory Rules (1989 Rules which have been created with a view to protect environment, nature and health in connection with the application of gene technology) clearly define Gene Technology as “the application of the gene technique called genetic engineering, including self-cloning and deletion as well as cell hybridisation” (Rule 3(iii)) while Genetic Engineering is defined as “the technique by which heritable material, which does not usually occur or will not occur naturally in the organism or cell concerned, generated outside the organism or the cell is inserted into said cell or organism. It shall also mean the formation of new combinations of genetic material by incorporation of a cell into a host cell, where they occur naturally (self cloning) as well as modification of an organism or in a cell by deletion and removal of parts of the heritable material” (Rule 3(iv)). Gene Editing of ALL kinds is clearly covered in these definitions and the very act of deregulating contradicts the constitution of India. 

In 2018 the European Court of Justice ruled that gene editing (which it called “new mutagenesis  techniques) was indeed GM, gives rise to GMOs, and falls under the scope of the EU GMO regulations. The court stated that the risks linked to the use of these new techniques might prove similar to those posed by older-style GMOs. The public scientific debate is still underway in Europe.

Apart from this, there are many scientific papers from other countries who have been researching gene editing in agriculture for longer and more independently than in India that point to the need for regulating Gene-editing technologies.

Gene Editing is Imprecise and all gene editing needs regulation

Gene editing is not precise. Numerous changes happen in the genome other than the change intended by the geneticist. These changes cannot be ignored because all genes have specific functions in a complex, well-regulated system and the outcomes in real life are unpredictable. The lack of precision means that there is greater unpredictability in varieties bred through this technique. It is often claimed that gene editing is equivalent to traditional breeding techniques, because the intended change does not involve the introduction of foreign DNA. 

Franziska Koller et al (2023)., and Marion Dolezel et al., conclude that when gene-editing is used to produce a new crop, the resulting gene combinations (intended or unintended) may be unlikely to occur with conventional methods and has the potential to affect both health and environment. The assessment of intended as well as unintended genetic changes should be part of a mandatory comprehensive molecular characterisation and risk assessment of gene-edited plants that are meant for environmental releases or for market authorisation.

Further, Katharina Kawall (2021) in her paper shows that small alterations in the genome of crop plants can nevertheless generate complex changes. The paper focuses on SDN-1 applications; it was found that nearly half of plants with so-called market-oriented traits contain complex genomic alterations induced by SDN-1 applications, which may also pose new types of risks. The results highlight the need for plants developed using new genetic engineering techniques to undergo case-specific risk assessment, taking both the properties of the end product and risks posed by the applied procedures into account.

Janet Cotter et al.,(2020) give an overview of the risks associated with gene-editing procedures for plants and animals. The risks are not only restricted to the wide range of unintended effects that can be triggered by the process of gene editing, but also with the intended biological characteristics generated through gene editing.

European Network of Scientists for Social and Environmental Responsibility (ENSSER), a large network of scientists put out a position paper in 2023 on how risks of gene editing techniques apply to SDN-1 as well (the same technique used for gene-edited rice in India). It also pointed out that unintended mutations still accumulate from the different stages and components of the gene editing process.

Eva Gelinsky and Angelika Hilbeck (2018), point out that advocates of the new genetic engineering techniques appear to believe that their benefits are so clear that furnishing reliable scientific evidence is unnecessary. This leads to oversight on the pitfalls of gene editing technology, and may lead to commercialization of gene-edited crops leading to irreversible damage to the environment, animals, and humans. 

Kosicki et al (2018) have shown that repair of double-strand breaks induced by CRISPR–Cas9 leads to large deletions and complex rearrangements.

In the face of such compelling scientific evidence, it is imperative that we subject gene-editing to the strictest of scrutiny. 

Imprecision of, and Unpredictable Changes from the so-called precise gene editing tool: 

To quote a couple of publications that show catastrophic unintended changes, Michael F. Eckerstorfer et al.,(2021) in their review show that gene editing is imprecise and  inaccurate, that changes brought by gene editing are different from those produced by conventional breeding, that gene-editing applications that don’t deliberately introduce “foreign DNA” are not by default safer than genetic engineering methods that do, the fact that some forms of gene editing are meant to only bring “small” changes in DNA is an unreliable denominator of risk/safety, that we possess very little and insufficient knowledge about the off-target modifications brought by some forms of gene-editing to assume safety, and finally the faster development of gene-edited plants is equal to higher  risk.

Further, Aviva Samach et al. (2023), in their paper show that chromothripsis, an extremely damaging form of genomic rearrangement that results from the shattering of individual chromosomes and the subsequent rejoining of the pieces in a haphazard order giving rise to “legitimate safety concerns” happens far more frequently in gene-edited crops than previously thought. It is important to note that chromothripsis refers to a phenomenon in which often several hundred genetic changes occur simultaneously in a catastrophic event. Many sections of the genetic material can be swapped, recombined, or even lost if this occurs. Therefore, evidence points to the fact that gene editing causes genetic changes that are different from those that happen in nature and consequences are poorly understood.

Unreliability of CRISPR/Cas application

There are several scientific papers cautioning us about the irreversible risks associated with Crisper technology, the same technology used in the two recently released gene-edited rice varieties. 

Ali Movahedi et al (2023) show that CRISPR/Cas9 technology raises serious biosafety concerns that require careful consideration, including potential off-target effects and the unintended transfer of modified genes to other organisms.  

Another paper by Katharina Kawall (2019) shows that in conventional breeding and spontaneous mutations, some regions in the genome undergo changes less frequently than others because these regions are especially protected by repair mechanisms in the cell. CRISPR/Cas applications can bypass these naturally occurring processes.

Presence of foreign genetic material

It is also important to note that de-regulated techniques of SDN-1 and SDN-2 gene editing also involve foreign genetic material being used and unintentionally implanted in the host organism.  It is often claimed that gene editing is equivalent to traditional breeding techniques, because the intended change does not involve the introduction of foreign DNA. But this is false, there are several research papers that falsify this claim. For instance, Jungeunet et al (2016) in their research show that foreign DNA and even complete foreign genes can be, and are, introduced into the genome by gene editing techniques, both intentionally and inadvertently. 

For example: Genome edited cattle – modified to have no horns – were found to have about 4000 kbp of superfluous DNA present originating from the bacterial plasmid vector, including two antibiotic resistance genes. This was not established by the producer of the cattle, but by researchers in the regulatory agency FDA. Norris et al. 2020, Nature Biotechnology 38, pages 163–164(2020), doi: 10.1038/s41587-019-0394-6 

Other cases of unintended integration of non-host DNA include: mice (e.g. Ono et al., 2015 and Ono et al. 2019); Plants (e.g. Jacobs et al., 2015, Li et al., 2015); fish (Gutierrez-Triana et al., 2018); fruitflies  (Drosophila melanogaster), nematodes (C. elegans), yeast and other fungi (eg Aspergillus); and planktonic crustaceans (Daphnia magna).

Whilst the insertion of vector DNA into DNA double-strand breaks is in itself a matter of concern, it is of perhaps even greater concern, that any other trace DNA present in the culture medium may be inserted into the host DNA. Ono et al., 2019, for example identified the presence of goat DNA and bovine DNA in the genome of the genome edited mice. This depended on whether goat or foetal calf serum had been used as a culture medium in the experiments. In fact, even retrotransposons had been transferred. In this context it becomes obvious that genome editing may unintentionally become a mechanism for horizontal gene transfer of not only foreign DNA but pathogens alike.

In a recent paper on CRISPR-cas9-mediated HDR  (Skryabin et al. 2020; Science Advances doi: 10.1126/sciadv.aax2941) authors reported not just about the unintended insertion phenomena (both for NHEJ & HDR) but also the difficulty of detecting these. The authors state in their abstract: “Nevertheless, the rapidly evolving technique still contains pitfalls. During the generation of six different conditional knockout mouse models, we discovered that frequently (sometimes solely) homology-directed repair and/or non-homologous end joining mechanisms caused multiple unwanted head-to-tail insertions of donor DNA templates. Disturbingly, conventionally applied PCR analysis, in most cases, failed to identify these multiple integration events, which led to a high rate of falsely claimed precisely edited alleles. We caution that comprehensive analysis of modified alleles is essential and offer practical solutions to correctly identify precisely edited chromosomes.”

Since SDN-1 and SDN-2 gene editing have been effectively de-regulated in India, there is no mechanism by which transparent confirmation (with reports placed in the public domain) can happen with apex regulatory bodies’ involvement (like the GEAC’s) to check whether foreign genetic material is present in the host organism.

High risk associated with gene-edited rice, Kamala and Pusa DST Rice 1 and gene-edited cattle

Evidence points to the fact that gene editing causes genetic changes that are different from those that happen in nature and their consequences are poorly understood. Chu P and Agapito-Tenfen  in their 2022 paper show that identifying certain larger DNA damages could only be possible using whole genome sequencing/long-read sequencing, which is generally not performed. For this and other reasons mentioned in the paper, Chu P et al. point out that the occurrence of unintended DNA damages is underestimated in the literature.

Sukumar Biswas et al. in their paper published in May 2020 conclude that SDN-1 technology, using CRISPR/Cas9 system is imprecise in rice, and that early and accurate molecular characterization and screening must be carried out for many generations. In many cases they found large insertions, deletions, and rearrangements of DNA, raising the possibility that the function of genes other than the one targeted could have been altered.

Also, Antibiotic Resistant Marker genes have been used for creating these mutants and they might be transmitted to other organisms through Horizontal Gene Transfer. There is a risk of antibiotic-resistance being transferred to humans through gene-edited rice. Norris et al (2020), have shown antibiotic resistance genes being transferred unintentionally in gene-edited cattle.

Hoepers et al (2024) show how gene editing has potential to cause unintended adverse impacts in non-target organisms too. 

In the face of such strong evidence, it is unsafe to release the two gene edited varieties of rice Kamala (DRR Dhan 100), developed by the Indian Institute of Rice Research (ICAR-IIRR), Hyderabad, and Pusa DST Rice 1, developed by the Indian Agricultural Research Institute (IARI), Delhi. They have been developed using SDN-1 technology, using CRISPR/Cas9 system, the exact same technology that Sukumar et al., show is imprecise for rice. In fact, reports have not been put out in the public domain to show that there are no safety concerns around these rice varieties.


Coalition for a GM-free India 

Website: www.indiagminfo.org, email : indiagmfree@gmail.com,  Facebook pageGM Watch India

 

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